Ovine fetal sex determination using circulating cell-free fetal DNA (ccffDNA) and cervical mucous secretions
نویسندگان
چکیده
منابع مشابه
Fetal sex determination using circulating cell-free fetal DNA (ccffDNA) at 11 to 13 weeks of gestation.
OBJECTIVE To examine the performance of a mass spectrometry-based detection platform using three Y-chromosome sequences for fetal sex determination from circulating cell-free fetal DNA (ccffDNA) in maternal blood in the first trimester of pregnancy. METHODS We extracted ccffDNA for the determination of fetal sex from stored maternal plasma obtained at 11 to 13 weeks' gestation from singleton ...
متن کاملearly non-invasive determination of fetal sex using cell-free dna
the first step in the prenatal diagnosis of x-linked genetic disorders is determining fetus gender. current invasive methods to obtain the dna source of the fetus instead of its miscarriage risk, has harmful stress for high risk pregnancies. cell free fetal dna (cffdna) circulating in the maternal blood, has now become a useful source of noninvasive prenatal diagnosis. considering limitation of...
متن کاملEvaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma
OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...
متن کاملPrenatal determination of farm animal fetal sex using free fetal DNA in maternal plasma
In many farm animal species, the preference of a specific sex is obvious mainly due to commercial implications; so for comprehensive and efficient results in the livestock management plans specially breedingand selection programs, it is necessary to include acost-effective and reliable strategy for early determination of fetal sex. Several invasive and non-invasive approaches have been establis...
متن کاملFetal sex determination via Nested-PCR method using Amylogenin gene
Background: Detection of fetal sex is valuable for parents. The aim of this study was detection of fetal sex in suspect cases recessive related to sex disease in first trimester of pregnancy. Material and method: After collecting C.V samples and separating from mother’s samples, their DNAs have been extracted, then doing PCR, finally the sex of them detected. Also, after increase in sensitivit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Asian Pacific Journal of Reproduction
سال: 2015
ISSN: 2305-0500
DOI: 10.1016/s2305-0500(14)60061-0